Eur. Phys. J. E (2009) 30: 149
https://doi.org/10.1140/epje/i2009-10520-9

Regular article

On diamond surface properties and interactions with neurons

¹ Nanostructured Surface and Interface Excellence Centre (NIS), University of Torino, Torino, Italy
² Department of Animal and Human Biology and CNISM-CNR, University of Torino, Torino, Italy
³ Department of Experimental Physics, University of Torino, Torino, Italy
⁴ Department of Physics, Polytechnic of Torino, Torino, Italy

^* e-mail: ettore.vittone@unito.it

Received: 22 April 2009
Revised: 10 September 2009
Accepted: 14 September 2009
Published online: 11 October 2009

Abstract

In this paper we report about the role the diamond surface morphology and atomic termination plays in the survival and viability of neuronal cells, which represent an appropriate experimental model for the development of cell-based biosensors. The samples we have investigated were both CVD homoepitaxial diamond films and nanocrystalline diamond layers deposited on quartz substrates. Different surface terminations were induced through exposure to atomic hydrogen and to intense UV irradiation. GT1-7 cells, a neuronal line of hypothalamic origin, were plated directly onto the diamond surfaces without exogenous adhesion molecules, in order to correlate the surface topography and chemistry to cell growth and viability. The cell density on nanocrystalline diamonds after 48h from plating was approximately 55% of the control on plastic dishes, whatever is the atomic termination of the surface, whereas the performances of homoepitaxial samples in terms of cell growth depend on surface termination and were significantly lower, 30%.-1