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Soft Matter and Biological Physics
Eur. Phys. J. E 10, 115-121 (2003)
DOI: 10.1140/epje/e2003-00013-2

Measuring a colloidal particle's interaction with a flat surface under nonequilibrium conditions

Total internal reflection microscopy with absolute position information
S.H. Behrens, J. Plewa and D.G. Grier

Dept. of Physics, James Franck Institute, and Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL 60637, USA


(Received 10 October 2002 Published online: 16 April 2003)

Abstract
A new and general approach is proposed to analyze the dynamics of a colloidal particle interacting with a nearby wall. This analysis can be used to determine the acting forces even when the system is non-stationary. As an illustration, we use total internal reflection microscopy to investigate the forces acting on a polystyrene sulfate latex particle as it is receding from a charged glass surface.

PACS
82.70.Dd - Colloids.
05.40.-a - Fluctuation phenomena, random processes, noise, and Brownian motion.

© EDP Sciences, Società Italiana di Fisica, Springer-Verlag 2003