https://doi.org/10.1140/epje/s10189-025-00516-0
Regular Article - Living Systems
Proportional modulation of proliferation and motility under 2D compressive stress depends on mesenchymal phenotype
1
LAAS-CNRS, CNRS, University of Toulouse, Toulouse, France
2
CRCT, Inserm, CNRS, University of Toulouse, Toulouse, France
a
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Received:
24
April
2025
Accepted:
11
August
2025
Published online:
3
September
2025
Abstract
Tumor development is accompanied by strong physico-chemical modifications. Among them, compressive stress can emerge in both the epithelial and stromal compartments. Using a simple two-dimensional compression assay which consisted in placing an agarose weight on top of adherent cells, we studied the impact of compressive stress on cell proliferation and motility in different pancreatic cancer cell lines. We observed a proportional reduction of both proliferation and motility in all tested cell types, with genotypes displaying a more “mesenchymal” phenotype (high velocity-to-proliferation ratio) and others related to a more “epithelial” phenotype (low velocity-to-proliferation ratio). Moreover, “mesenchymal” cells seemed more sensitive to compression, a result that was further suggested by a TGF
1 induction of epithelial-to-mesenchymal transition. Finally, we measured that the change in cell proliferation was associated with a change in intracellular macromolecular crowding, which could modulate a plethora of biochemical reactions. Our results together suggest a mechanism in which all biochemical reactions related to proliferation and motility can be modulated by a change in macromolecular crowding, itself depending on the phenotype, leading to differential sensitivity to pressure.
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© The Author(s), under exclusive licence to EDP Sciences, SIF and Springer-Verlag GmbH Germany, part of Springer Nature 2025
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
